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Literature summary extracted from

  • Christian, E.L.; Kaye, N.M.; Harris, M.E.
    Helix P4 is a divalent metal ion binding site in the conserved core of the ribonuclease P ribozyme (2000), RNA, 6, 511-519.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.26.7 additional information construction of 2 self-cleaving ribozyme-substrate conjugates for identification of nucleotides involved in catalysis Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.26.7 additional information
-
 additional information effects of phosphorothioate substitutions on ribozyme catalysis, kinetics, overview Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.26.7 Mg2+ 25 mM, required for folding and catalytic function, metal binding site and coordination study using site-specific phosphorothioate substitutions, helix P4: the pro-Sp phosphate of Ala67 is involved in metal ion positioning Escherichia coli
3.1.26.7 NaCl
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.26.7 pre-tRNA + H2O Escherichia coli the helix P4 is important in the ribozyme for positioning divalent metal ions that are required for catalysis tRNA + pre-part nucleotides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.7 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.7 pre-tRNA + H2O
-
 Escherichia coli tRNA + pre-part nucleotides
-
 ?
3.1.26.7 pre-tRNA + H2O the helix P4 is important in the ribozyme for positioning divalent metal ions that are required for catalysis Escherichia coli tRNA + pre-part nucleotides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.26.7 More ribozyme secondary structures Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.26.7 ribonuclease P ribozyme
-
 Escherichia coli
3.1.26.7 RNase P RNA
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.7 50
-
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.7 5.5
-
-
 Escherichia coli